Buellia sublauri-cassiae
Buellia sublauri-cassiae A.H. Ekanayaka & K.D. Hyde, in Ekanayaka, Jones, Zhao & Hyde, Asian Journal of Mycology 2(1): 63 (2019)
Buellia sublauri-cassiae Ekanayaka & K.D. Hyde, sp. nov.
Index Fungorum number: IF556242; Facesoffungi number: FoF05804
Etymology – refers to the similarity to Buellia lauricassiae
Holotype – MFLU 18-0672
Lichenized on dead stem. Thallus: crustose, yellowish to grey, granulose, rimose. Sexual morph: Apothecia 400–800 mm diam., arising singly or in small groups, sessile, slightly erumpent from the thallus, pulvinate, roundish or irregular, black. Hypothecium convex. Disc smooth to slightly granulose, black. Margins distinct, black. Hymenium hyaline, within a thick gelatinous matrix. Epithecium branched, swollen and pigmented paraphyses apices form clearly distinguishing pseudo epithecium above the hymenium, brown. Excipulum 40–50 µm at flanks, composed of small, thin-walled, cells of textura intricata without algal cells, inner cells are hyaline to brownish, outer cells are brown, with orange pigments. Paraphyses 1.2–1.8 µm wide at the middle (x = 1.4 µm, n = 20), 2–4.5 µm wide at the tip ( x = 3.2 µm, n = 20), numerous, filiform, propoloid, aseptate, branched, swollen and pigmented at the apices. Asci 50–75 × 10–20 µm (x = 60 × 15 µm, n = 30) 8-spored, narrowed to base, short pedicel, cylindric–clavate, rounded at the apex, nonamyloid. Ascospore 15–20 × 7–9 µm (x = 17 × 8 µm, n = 40), greenish brown, smooth walled, ellipsoid, tear-shaped, lover cell is slightly wider than upper cell, lower apex rounded, upper apex slightly pointed to rounded. Asexual morph: Undetermined.
Material examined – Thailand, Chiang Rai Province, Mae Fah Luang University, on dead stem, 28 June 2016, A.H. Ekanayaka HD04Y (MFLU 18-0672, holotype); same collections details (HKAS 104246, isotype); Thailand, Chiang Rai Province, Mae Fah Luang University, on dead stem, 12 November 2017, A.H. Ekanayaka HD064 (MFLU 18-0681, paratype)
GenBank accessions – ITS: MFLU 18-0672- MK499343, MFLU 18-0681- MK499344; LSU: MFLU 18-0672- MK499355, MFLU 18-0681- MK499356
Notes – Our new collection from Thailand clustered with Buellia with high (100%) bootstrap support (Fig. 9). Phylogenetically Buellia sublauri-cassiae groups with Buellia lauri-cassiae with high statistical support (100%), but differs by 54 base pairs (ITS) from Buellia lauri-cassiae. Moreover, Buellia sublauri-cassiae differs from Buellia lauri-cassiae by having 1-septate ascospores (Watanuki et al. 2017). Morphological characters of our collection are similar to Buellia halonia (Bungartz et al. 2004), but differ in not having areolate greenish thallus black in outline.
Morphology of Buellia sublauri-cassiae (holotype MFLU 18-0672). a Substrate. b Ascomata on wood. c Cross section of an ascoma. d Close up of a vertical section of the ascoma at margin. e Aseptate paraphyses. f, g Short pedicellate asci. h-j Ellipsoid ascospores. Scale bars: c = 100 µm, d = 40 µm, e-g = 20 µm, h-j = 10 µm
Phylogram generated from a maximum likelihood analysis based on ITS sequence data. The newly generated nucleotide sequences were compared against the GenBank (http://www.ncbi.nlm.nih.gov/) database using the Mega BLAST program. Related sequences were obtained from GenBank. One hundred thirty three strains were included in the sequence analyses, which comprised 640 characters including gaps. Lecanora contractula AFTOLID 877 was used as outgroup. The best scoring RAxML tree with a final likelihood value of - 18531.023484 is presented. The matrix had 513 distinct alignment patterns, with 20.64 % of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.219, C = 0.289, G = 0.261, T = 0.231; substitution rates AC = 1.437028, AG = 2.537533, AT = 1.696164, CG = 0.808811, CT = 6.126467, GT = 0.474678; gamma distribution shape parameter α = 1.381352. Bootstrap support values for ML equal or greater than 50% are given above the nodes. Newly generated sequences in blue.
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