Cistella granulosella

Cistella granulosella (P. Karst.) Nannf, IF: 266483,Facesoffungi number: FoF 05938
Saprobic on dead stems. Sexual morph: Apothecia 130–180 μm wide, arising singly or in small groups, sessile, erumpent from the substrate, brownish white when fresh. Receptacle cupulate to urceolate. Disc concave, disc and the margins are brownish white when fresh. Hairs 25–32 × 3.5–5 μm ( x = 30.3 × 4 μm, n = 30), cylindric, clavate, 1–2-septate, hyaline, walls usually thin, apices slightly swollen and finely granulate. Ectal excipulum 18–25 μm ( x = 21.8 μm, n = 10) in margins and upper flanks, 3–4 cells deep, cell walls are thick, brown cells of textura globulosa to angularis. Medullary excipulum 12–22 μm (x = 16.3 μm, n = 10) in upper flanks, composed of narrow, long, thin-walled, hyaline cells of textura epidomoidea. Hymenium hyaline. Paraphyses 1.2–1.8 μm wide ( x = 1.4 μm, n = 20), numerous, filiform, branched, septate, hyaline, acute at the apex, not exceeding asci in length. Asci 30–40 × 5–7 μm ( x = 33.4 × 5.7 μm, n = 30), 8-spored, unitunicate, cylindric-clavate, rounded or medium conical and amyloid apex, short stipitate base, arising from croziers. Ascospores 6–7.5 × 1.5–1.8 μm (x = 7 × 1.7 μm, n = 40), multi-seriate, ellipsoid-clavate, aseptate, hyaline, one side rounded and other side slightly pointed. Asexual morph: Undetermined.
Material examined – UK, Isle of Wight, Calbourne stream, on Apiaceae stem, 11 May 2015,
E. B. G. Jones, GJ154a (MFLU 16-0565).
GenBank accessions – LSU- MK591962, ITS- MK584936, SSU- MK585023
Notes – Our new collection from UK grouped with Cistella albidolutea (KUS F52678) and C. grevillei, but with low statistical support (Fig. 51). The ITS data of our collection is similar to Polyphilus sieberi (strain 17A) (517/551-94% with 8 gaps), Urceolella carestiana (TNS-F18014) (491/526-93% with 6 gaps), Cistella albidolutea (KUS-F52678) (480/526-91% with 11 gaps) and to Leohumicola minima (CBS 209.74) (492/535-92 % with 9 gaps). The LSU region is similar to that of Polyphilus frankenii (strain V16) (839/862-97% with 1 gap), Polyphilus sieberi (strain M03) (840/862-97% with 1 gap) and to Tetracladium maxilliforme (CCM F-13186) (1080/1176-92% with 15 gaps). Cistella granulosella is similar to Psilachnum and Cistella, but differs from Psilachnum by having hairs with granulate walls. However, its morphology fits with the description of Cistella granulosella provided by Raitviir (2004). Cistella granulosella is similar to C. dentata but differs in lacking dentate margins and slightly smaller asci (Quijada et al. 2015).