Erioscyphella sclerotii
Erioscyphella sclerotii (A.L. Sm.) Baral, Šandová & B. Perić
= Lachnum sclerotii (A.L. Sm.) J.H. Haines & Dumont
Facesoffungi number: FoF 05907; Fig. 38
Saprobic on dead stems. Sexual morph: Apothecia 0.6–1 × 1.2–1.5 mm, arising singly or in small groups, superficial, stipitate, yellow when fresh, covered with long, brown hairs. Receptacle cupulate, disc concave and bright yellow, margins and flanks brown. Stipe hairy, medium to long in length. Hairs 35–60 × 3–5 µm (47 × 3.7 µm, n = 30), on flanks and stipe, cylindrical to blunt hemispherical tip, straight, thin-walled, septate, brown, densely covered with colorless granules. Ectal excipulum 35–40 µm wide (x = 37.3 µm, n = 10) in lower flanks, composed of brownish cells textura prismatica. Medullary excipulum 78–85 µm wide (x = 82 µm, n = 10) in lower flanks, with few cell layers comprising hyaline cells of textura intricate, outer cells are tightly packed and inner cells are loosely packed. Hymenium hyaline. Paraphyses 2–2.5 µm wide (x = 2.3 µm, n = 10), numerous, filamentous, septate. Asci 70–80 × 6–7 µm (x = 78.9 × 6.6 µm, n = 30), 8-spored, unitunicate, cylindrical, inoperculate, amyloid, short-stipitate base, sub-stipitate base. Ascospore 23–27 × 2.5–3 µm (x = 25.2 × 2.8 µm, n = 40), biseriate, fusiform, hyaline, 3-septate, smoothwalled, no sheath or appendages, sometimes with refractive inclusions. Asexual morph: Undetermined.
Material examined – Thailand, Chiang Rai Province, Mae Sai District, Pongpha, Tham Pla Temple M.14 (Bann Tham Pla Temple), on dead stem, 25 November 2014, A. H. Ekanayaka, HD002 (MFLU 16-0569), Thailand, Chiang Rai Province, Kun Korn water fall, 12 January 2018, A. H. Ekanayaka, HD72 (MFLU 18-0688).
GenBank accessions – MFLU 16-0569: LSU- MK591980, ITS- MK584951, SSUMK585033, RPB2- MK388219; MFLU 18-0688: LSU- MK591995, ITS- MK584969, SSUMK585042, RPB2- MK614726
Notes – Our two collections from Thailand grouped with Erioscyphella sclerotii (Fig. 35) and the clade received strong statistical support of 98%. The ITS data of our collection shows similarity to Lachnum sclerotii (strain 3188) (528/534-99% with 2 gaps). The LSU data of our collections is similar to L. abnorme collections, TNS:F-16617 (1080/1102-98% with 1 gap) and KUS-F52080 (1077/1103-98% with 3 gaps). Erioscyphella sclerotii is characterized by having stipitate, cupulate, yellow apothecia, granulate cylindrical hairs, fusiform 3-septate ascospores (Smith 1901). Erioscyphella abnormis is similar to E. sclerotii but it has 7-septate ascospores (Spooner 1987).
Figure 38 – Morphology of Erioscyphella sclerotii (MFLU 16-0569) a, b Apothecia in wood. c Apothecium in wood. d Cross section of a apothecium. e Close up of the peridium. f Setae on apothecium. g Septate seta in water. h–k Asci in water. l Septate paraphyses in water. m Asci in Melzer’s reagent with J+ discoid apical apparatus. n–q Fusiform ascospores in water. r Germinated ascospore. Scale bars: a = 500 µm, b = 20 µm, c = 1000 µm, d = 400 µm, e = 50 µm, f–l = 25 µm, m = 5 µm, n–q = 10 µm, r = 25 µm.
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