Allophylaria subliciformis

Allophylaria subliciformis (P. Karst.) P. Karst. IF: 204044
≡ Peziza subliciformis P. Karst. [as ‘sublicoides’], Not. Sällsk. Fauna Fl

Ascocarps (0.15–)0.25–0.5 mm in diam., gregarious in large numbers, whitish to pale yellow, goblet–shaped, and with a small cylindrical stipe of 0.1-0.2 mm height and 0.1-0.15(–0.17) mm width, pale yellow- to grey-brown in the upper part and darker brown below, superficial. Asci *(140–)150–190(–240) × (15–)15.5–17.5(–19) μm {5}, †135–170 × (10–)11–14(–15) µm {3}, cylindric-clavate, narrowed towards the base, apex with strongly hemiamyloid apical ring of Laetinaevia type {6}, blue at low and red at high iKi concentration (rb), or sometimes almost directly red (rr), negative in Mlz but deep blue in iKi or Mlz when Koh-pretreated, with croziers {4}, 8-spored, spores obliquely biseriate, pars sporifera *58–65 µm. Ascospores *(19.5–)21–26(–30) × (6–)6.5–7(–7.5) μm {6} (Q = 3.1–4.1, n = 68, r.M.), †(17–) 19–23(–24) × 5–6.3 µm {2}, cylindric-ellipsoid, ends obtuse to almost subacute, homopolar, inequilateral to mostly slightly (to medium) curved (allantoid), usually non-septate within living asci but sometimes also 1-septate {1}; overmature with 1–3 septa, scarcely constricted at the septa; surface pale lilac in Crb due to a thin gel layer; lipid bodies (2–)3–4(–4.5) µm diam., 1(–3) in each half, surrounded by many small ones (lipid content 4.5–5); in septate spores with one lb of 4–5.5 µm diam. in each cell; freshly ejected spores covered with a delicate sheath that soon detaches and is then difficult to see; sometimes forming chalara-like phialides on the spores. Paraphyses filiform, slightly thickened at the cylindric-clavate apex to *(2–)3–5(–5.7) μm, often somewhat curved, rarely antler-like, septate, terminal cell *(28–)32–41(–44) µm in length, containing one or a few large, hyaline to pale yellowish vacuolar bodies that occupy almost all its space, reaching 10–20 µm from top, Vbs disintegrating into small, orange-yellow to red-brown drops in the presence of iKi. Medullary excipulum hyaline, formed of long cylindrical cells 2–4 μm diam. Ectal excipulum in receptacle hyaline, of textura porrecta to porrecta-oblita, composed of cylindrical cells in parallel and unbranched, partly undulating disposition, cells *3–8 μm wide, moderately thick-walled (strongly so in dead state), more thinwalled with age, agglutinated by a gelatinous outer wall layer that swells in dead state; margin formed by chains of cylindrical cells, terminal cells cylindrical to claviform, *12–24 × 4–8 μm, containing Vbs similar to the paraphyses; stipe brown (in Koh olive-brown), sometimes with yellowish exudate.

Holotype (fide hUhtinen, 1990: 242): [Finland, tavastia australis,] Mustiala, ad Art.[emisia], 31.X.1867, P.a. Karsten (h, herb. Karsten 884, not examined).

Specimens included : SPAIN. Community of La Rioja, 14 km sW of logroño, 0.2 km se of hornos de Moncalvillo, 42° 23’ 22’’ n – 2°35’ 1’’ W, 683 m alt., on unidentified herbaceous dicot stem, 3.Xii.2016. leg. l. ballester, herb. r.M. 2374, Genbank Mh221035 – GERMANY. Mecklenburg–Vorpommern, 20 km WnW of schwerin, 1.5 km n of Pokrent, recultivated sand pit, ~53° 39’ 40’’ n – 11° 8’ 15’’ e, 60 m alt., stem of Melilotus, 13.Xi.2010, leg. t. richter, herb. h.b 9456a. – Thüringen, 2.5 km s of sonneberg, 1 km WsW of oberlind, an der Müß, recultivated gravel pit, 50° 20’ 12’’ n – 11° 9’ 55’’ e, 358 m alt., stem of Solidago canadensis, 12.Xi.2015, leg. i. Wagner (unpreserved, illustrations seen). – Rheinland–Pfalz, 1.7 km nnW of neustadt a.d. Weinstr., haardt, 49° 21’ 47’’ n – 8° 8’ 17’’ e, 195 m alt., stem of indet. herbaceous dicot, 7.Xii.1975, leg. r. thate, herb. h.b. 622. – Baden–Württemberg, 5 km ne of tübingen, 0.6 km s of Pfrondorf, obere Mähder, 48° 32’ 36’’ n – 9° 6’ 40’’ e, 390 m alt., fruit of Oenothera biennis, 14.i.1990, leg. h.-o. baral (unpreserved, 1 apothecium). – CANADA. Prince-Edward Island, 8 km ssW of Charlottetown, Cumberland, 32 m alt., inflorescence of Cirsium, 30.X.2015, leg. a. Carter, a.C. 2168

Fig. 1 – Allophylaria subliciformis (r.M. 2374, photos r. Martínez-Gil). a: habitat of the spanish collection, arrow indicating the exact position where the apothecia were found. b, C: Fresh apothecia, in situ. D: apothecia in the dry state (after 13 months in the herbarium). e–F: Do., margin. G: Medullary excipulum (squash mount). h: ectal excipulum at flanks in external view. all in living state. (e, G, h in water, F in iKi). scale bar = 20 μm for e–h.